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KMID : 0613820000100040397
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Journal of Life Science
2000 Volume.10 No. 4 p.397 ~ p.402
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Characterization of Polyphosphate Kinase Gene in Serratia marcescens
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Choi Yang Lark
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Abstract
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Polyphosphate kinase catalyzes the formation of polyphosphate from ATP. To understand the mechanism of phosphate accumulation, the Serratia marcescens gene encoding ppk was cloned from the genomic library by the method of Southern hybridization. The hybridization positive DNA fragment region from pDH3 was subcloned into the expression vector. The ppk gene product, a polypeptide of 75 kDa, was confirmed by SDS-PAGE. Expression of the Serratia marcescens ppk is regulated by the catabolite repression system. The enzyme activity polyphosphate kinase was increased in the E. coli strain harboring plasmid pMH4 with ppk gene.
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